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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant position in marketing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guidebook outlines more possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...
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This guide outlines even further achievable optimization of cellular and immunoassay parameters to be certain the best possible effects are...
Antibodies bound to the blocking peptide no more bind for the epitope over the target protein. By comparing the staining through the blocked antibody compared to the antibody on your own, you can see which staining is distinct.
Concentrate on Details Cyclin E belongs into the remarkably conserved cyclin loved ones, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which lead for the temporal coordination of each and every mitotic party. Cyclin E forms a fancy with and features being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S stage boundary which is degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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